Job Offer: Laboratory Technician (Yeast Genetics & Molecular Biology)

Contract duration: Minimum 1 year (with possibility of extension)

Start date: Flexible but no later than 1st June 2026

Workplace: Mar Albà research group (albagroup.researchmar.net), Hospital del Mar Research Institute, Barcelona Biomedical Research Park (PRBB)

About the Position

We are seeking a highly motivated and skilled Laboratory Technician to join our research group focused on yeast functional genomics. Our lab has recently identified a large set of previously unannotated, potentially novel genes in Saccharomyces cerevisiae using deep RNA-seq and Ribo-seq experiments. The successful candidate will play a central role in experimentally characterizing these genes by generating and validating gene knockout mutants across multiple yeast strains.

Main Responsibilities

• Construct gene deletion mutants in S. cerevisiae using homologous recombination–based gene disruption methods.

• Design and assemble DNA constructs (PCR, cloning, primer design).

• Perform yeast transformations and confirm successful knockouts (PCR, sequencing, phenotypic verification).

• Maintain strain collections, plasmid stocks, and accurate laboratory records.

• Carry out growth assays under a range of stress conditions (e.g., temperature, osmotic, oxidative, nutrient deprivation) to assess the functional relevance of the targeted genes.

• Analyze growth data to identify phenotypic effects linked to gene deletions.

Required Qualifications

• Bachelor’s or Master’s degree in Molecular Biology, Genetics, Biotechnology, or a related field.

• Hands-on experience with Saccharomyces cerevisiae genetics or microbial model organisms.

• Strong skills in molecular techniques such as PCR, cloning, gel electrophoresis, and DNA preparation.

• Ability to work independently, manage multiple constructs/strains in parallel, and maintain detailed lab documentation.

Preferred Qualifications

• Experience with homologous recombination–mediated gene deletions in yeast.

• Experience performing growth assays or phenotyping under stress conditions.

• Familiarity with next-generation sequencing workflows or genomic analysis.

• Interest in gene function, genome annotation, or non-canonical translation.

What We Offer

• A full-time position for at least one year, with the possibility of renewal. Salary will follow our institution’s pay structure and will be adjusted according to your experience.

• Opportunity to contribute to cutting-edge research on novel genes and genome biology in the context of an ERC Advanced Grant project.

• Supportive, collaborative working environment with training opportunities.

• Access to state-of-the-art molecular biology and functional genomics facilities.

How to Apply

Please send your application including:

• A brief motivation letter

• CV

• Contact information for at least one reference

to: [email protected]